Poster Presentation Society for Molecular Biology and Evolution Conference 2016

Genome-wide expression profiling analysis identifies aging-associated gene expression change (#450)

Jae-Won Huh 1 2 , Ja-Rang Lee 1 , Se-Hee Choe 1 2 , Hyeon-Mu Cho 1 2 , Sang-Je Park 1 , Young-Hyun Kim 1 2 , Sang-Rae Lee 1 2 , Kyu-Tae Chang 1 2
  1. National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Chungbuk, Republic of Korea
  2. University of Science & Technology (UST), National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju , Chungbuk, Republic of Korea

Chlorocebus aethiops is an old world monkey, has been traditionally used as an experimental model for biomedical research. In addition, recent technological advances of next generation sequencing is useful for unravel the genetic mechanisms of senescence, aging and age-related diseases. Here we analyzed the blood transcriptome of aged C. aethiops from nine individuals ranging in age from 17 to 25 years old over the next year using the Illumina sequencing platform. We identified 418 to 676 differentially expressed genes (DEGs) in each nine individuals which tend to increase with age. Among the DEGs, 35 genes were differentially expressed between two time points in nine individuals commonly. A major proportion of the common 35 DEGs were belonged to gene ontology categories involved in translation, translational elongation, and regulation of cellular protein metabolic process. Additionally, we added third-year blood samples, and 13 common DEG s were experimentally confirmed. This study reported the gene expression changes during aging C. aethiops transcriptome, and common genes identified have the potential as the particular target of aging.