Genetic complexity of an organism studies aimed at defining the structure of gene and determining the processes responsible for how gene expression at the transcripts and protein levels are regulated have led to regulatory mechanism. And also, insertion of transposable element into the promoter region could affect the transcription of cellular genes. The main aim of our study was to find antisense transcripts in human genome that derived from the transposable elements. Using a bioinformatics approach, we searched the antisense transcripts derived from the transposable elements using the full-length cDNA sequence within human genome. In this study, we established a set of very stringent criteria to identify the correct orientation of each transcript. From our in silico analysis of human genome indicated that 423 antisense transcripts gene pair was identified to have been affected by transposable elements during the cellular gene expression. The large number of sense-antisense transcripts suggests that gene regulation by antisense transcripts derived from the transposable elements in human genome. Antisense transcripts regulate of transposable elements may also provide understanding of the complex regulation networks and dynamic evolutionary features during human evolution.