Poster Presentation Society for Molecular Biology and Evolution Conference 2016

Selective breeding mediated genetic mapping for tameness in mouse (#644)

Yuki Matsumoto 1 2 3 , Jo Nishino 4 , Tatsuhiko Goto 3 5 , Tsuyoshi Koide 1 2
  1. Department of Genetics, SOKENDAI, Mishima, Shizuoka, Japan
  2. Mouse Genomics Resource Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan
  3. JSPS, Chiyoda-ku, Tokyo, Japan
  4. Graduate School of Medicine, Nagoya University, Nagoya, Aichi, Japan
  5. School of Life Sciences, The University of Nottingham, University Park, Nottingham, United Kingdom

Tameness is a major behavioral factor for the domestication. It can be divided into two potential components: motivation to approach humans (active tameness) and reluctance to avoid them (passive tameness), and we previously established behavioral assay for the quantification of the both types of tameness in mice (Mus musculus). To identify genes associated with active tameness, we performed selective breeding for contacting (defined as contacting human hand) using wild-derived heterogeneous stock (WHS). WHS is a mixed population derived from 8 wild mouse strains. At the 8th generation of the selective breeding, contacting in selected population increased although control group did not. Then by using GigaMUGA SNP genotyping array, we obtained 20,530 single nucleotide polymorphism (SNP) data of 32 mice in both selected and control population, and the 8 founder strains of WHS. Because the alleles associated with contacting should be increased in frequency by selective breeding, the selected loci can be identified by using the deviation from hypothetical allele frequency determined by computer simulation. We used the simulation based on non-selection model combined the pedigree information, genotype and the SNP position. Then we determined genome-wide thresholds for significant increase of allele frequency, and applied the threshold to observed data in the populations. As a result, we found a SNP on chromosome 11 exceeded the threshold. By using database, we will discuss about candidate genes within the detected SNP and the results of comparative genomics between mouse and dog, which is one of the major domesticated animal and shows tameness.