Speciation occurs when two populations split and accumulate genetic differences, which can lead to the evolution of reproductive isolating mechanisms. Species of the Drosophila genus remain some of the best models for the study of speciation. In spite of the power of Drosophila, little is known about the genetic basis of female mate preference. Previous work identified a pair of recently diverged races within Drosophila melanogaster that show strong behavioral isolation but little reproductive isolation of any other type. We leveraged the power of evolutionary genetics in Drosophila to dissect the genetic basis of the trait and identified to alleles involved in female choice.