Oral Presentation Society for Molecular Biology and Evolution Conference 2016

Patterns of adaptive evolution: a structural perspective (#62)

Greg Slodkowicz 1 , Nick Goldman 1
  1. EMBL-European Bioinformatics Institute, Cambridge, UNITED KINGDOM, United Kingdom

Protein structure is a major cause of site-to-site evolutionary rate variation. Many structural features such as solvent accessibility, local packing density and proximity to active sites or interfaces have been shown to modulate the evolutionary rate. It is, however, not well understood how these features affect the prevalence of adaptive evolution. Most codon-based models, which are commonly applied for detecting sites under positive selection, do not incorporate any information about the protein structure.

In this study, we attempted to form a better view of adaptation on molecular level by asking whether residues under positive selection are close to each other on the protein structure. We generated a large dataset of trees and alignments for 39 mammalian species (covering over 80% of human genes) and calculated sitewise values of selective constraint (dN/dS). We then mapped positively-selected sites onto available crystal structures and analysed whether they tend to be co-located by statistically assessing the distribution of pairwise distances between them.

We find that positively-selected sites frequently form tight clusters on protein structures and that this conclusion is robust to low alignment quality and other technical issues. Identified clusters can be assigned into one of several categories: we find that groups of positively-selected residues can surround active sites, occur in binding regions, and form small, linear clusters in the N-termini of proteins. To our knowledge, the last of these findings has not been previously reported. Additionally, the prevalence of clustering varies in different enzyme classes, with oxidoreductases exhibiting the most evidence for clustering.